843Enhancing Pathogen Identification Using a Comprehensive PCR System in Adult and Pediatric Patients with Meningitis and a Negative Gram Stain
نویسندگان
چکیده
System in Adult and Pediatric Patients with Meningitis and a Negative Gram Stain Susan Wootton, MD; Elizabeth Aguilera, MD; Lucrecia Salazar, MD; Andrew Hemmert, PhD; Rodrigo Hasbun, MD, MPH; Pediatrics, University of Texas Health Science Center, Houston, TX; Division of Infectious Diseases, University of Texas Medical School at Houston, Houston, TX; BioFire Diagnostics, LLC, Salt Lake City, UT; University of Texas Health Science Center at Houston, Houston, TX
منابع مشابه
Rapid diagnosis of bacterial meningitis by real-time PCR and fluorescence in situ hybridization.
Real-time PCR and fluorescence in situ hybridization (FISH) were evaluated as rapid methods for the diagnosis of bacterial meningitis and compared to standard diagnostic procedures. For PCR, a LightCycler approach was chosen, implementing eubacterial and specific PCR assays for the most relevant bacteria. For FISH, a similar probe set containing eubacterial and specific probes was composed of p...
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BACKGROUND Although cerebrospinal fluid (CSF) culture is the diagnostic reference standard for bacterial meningitis, its sensitivity is limited, particularly when antibiotics were previously administered. CSF Gram staining and real-time PCR are theoretically less affected by antibiotics; however, it is difficult to evaluate these tests with an imperfect reference standard. METHODS AND FINDING...
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